4ZNG
X-ray crystallography of recombinant Lactococcus lactis prolidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-11 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 212.130, 76.990, 88.920 |
Unit cell angles | 90.00, 112.39, 90.00 |
Refinement procedure
Resolution | 49.830 - 2.250 |
R-factor | 0.2227 |
Rwork | 0.220 |
R-free | 0.27950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wy2 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.830 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.084 | 0.800 |
Number of reflections | 63058 | |
<I/σ(I)> | 9.48 | 1.74 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | calcium acetate, sodium cacodylate, PEG 8000, glycerol |