4ZN3

Crystal Structure of MjSpt4:Spt5 complex conformation B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	45.395, 88.943, 91.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.22885
Rwork	0.227
R-free	0.25569
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zn1
RMSD bond length	0.006
RMSD bond angle	1.172
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge		0.480
Number of reflections	17139
<I/σ(I)>	15.5	3.3
Completeness [%]	99.4	100
Redundancy	4.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	PEG 6000, MPD, MES