4ZN0
Structure of the NADPH-dependent thioredoxin reductase from Methanosarcina mazei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.144, 182.500, 152.046 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.889 - 2.600 |
| R-factor | 0.2181 |
| Rwork | 0.217 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cty |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.737 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.890 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.102 | 1.196 |
| Number of reflections | 50353 | |
| <I/σ(I)> | 29.49 | 2.27 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Na Acetate pH 4.8, 1.00 M NH4Cl |
