4ZMQ
Crystal structure of human P-cadherin (ss-X-dimer)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-27 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 63 |
Unit cell lengths | 182.090, 182.090, 41.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.720 - 2.200 |
R-factor | 0.18861 |
Rwork | 0.186 |
R-free | 0.23112 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zmn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.603 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.720 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.078 | 0.387 |
Number of reflections | 37841 | |
<I/σ(I)> | 18.2 | 4.4 |
Completeness [%] | 94.6 | 70.7 |
Redundancy | 9.2 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 28% PEG 400 250 mM CaCl2 HEPES 100 mM |