4ZMJ
Crystal Structure of Ligand-Free BG505 SOSIP.664 HIV-1 Env Trimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-29 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 |
Unit cell lengths | 107.180, 107.180, 103.060 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.083 - 3.310 |
R-factor | 0.2676 |
Rwork | 0.266 |
R-free | 0.28550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tvp |
RMSD bond length | 0.004 |
RMSD bond angle | 1.166 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.360 |
High resolution limit [Å] | 3.310 | 3.300 |
Rmerge | 0.089 | 0.500 |
Number of reflections | 7041 | |
<I/σ(I)> | 18.5 | 1.16 |
Completeness [%] | 68.3 | 11.2 |
Redundancy | 5.2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 26% PEG 400, 3.2% PEG 3350, and 0.1M sodium acetate pH 5.5 |