4ZLR

Structure of the Brat-NHL domain bound to consensus RNA motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9184
Spacegroup name	P 41 2 2
Unit cell lengths	70.190, 70.190, 279.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.765 - 2.300
R-factor	0.1891
Rwork	0.188
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q7f
RMSD bond length	0.003
RMSD bond angle	0.754
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.765	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	32238
<I/σ(I)>	11.77	2.42
Completeness [%]	99.7	100
Redundancy	12.04	12.27

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	2 M Ammonium Sulfate, 5 % PEG 400, 100 mM MES