4ZLK

Crystal structure of mouse myosin-5a in complex with calcium-bound calmodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	56.079, 99.455, 248.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.849 - 2.502
R-factor	0.1989
Rwork	0.196
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oe9
RMSD bond length	0.009
RMSD bond angle	1.157
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Rmerge	0.106
Number of reflections	47736
<I/σ(I)>	2
Completeness [%]	97.4
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Tris-HCl, pH7.5, 2 mM CaCl2, 2 mM DTT, 2 mM NaN3, 10% glycerol, 6% PEG 8000