4ZKQ

Viral chemokine binding protein R17 encoded by rodent gammaherpesvirus Peru ( RHVP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	69.561, 75.835, 106.985
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.011 - 1.898
R-factor	0.1862
Rwork	0.184
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The 2.7 Angstrom model resolved by SAD experiment
RMSD bond length	0.005
RMSD bond angle	0.996
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	REFMAC
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.890	1.890
Rmerge		0.650
Number of reflections	45516
<I/σ(I)>	20.8	1.83
Completeness [%]	93.8	61.2
Redundancy	3	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1M Tris pH 8.5 20% PEG550 MME