4ZKI
The crystal structure of Histidine Kinase YycG with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.499, 96.034, 119.351 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.819 - 3.401 |
R-factor | 0.2025 |
Rwork | 0.201 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u7o |
RMSD bond length | 0.011 |
RMSD bond angle | 1.437 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.400 |
Number of reflections | 8921 |
<I/σ(I)> | 10 |
Completeness [%] | 99.8 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 298 | 1.0 M Ammonium Sulfate, 1% PEG 3350 |