4ZJL
Crystal structure of AcrB in complex with antibiotic in P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 150.948, 155.665, 217.007 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 19.974 - 3.470 |
| R-factor | 0.2545 |
| Rwork | 0.251 |
| R-free | 0.32130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gif |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.801 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.520 | 3.670 |
| High resolution limit [Å] | 3.470 | 3.470 |
| Number of reflections | 127313 | |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 98.7 | 96.1 |
| Redundancy | 4.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M MES pH 6.5, 0.2 M MgAc, 10% PEG 3350 |
