4ZJA
Small heat shock protein AgsA from Salmonella typhimurium: C-terminal truncated construct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 126.400, 126.400, 126.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.689 - 4.101 |
| R-factor | 0.387 |
| Rwork | 0.386 |
| R-free | 0.41270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zj9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.539 |
| Data reduction software | MOSFLM (7.0.9) |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.200 | 4.320 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.010 | |
| Number of reflections | 2757 | |
| <I/σ(I)> | 14.8 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% Pentaerythritol propoxylate (5/4 PO/OH), 0.1M MES-NaOH, 30% glycerol |
