4ZIZ
Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase (C-Phycocyanin from T. elongatus)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2014-02-06 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.56 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 186.380, 186.380, 60.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.561 - 1.750 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l0f |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.702 |
| Data reduction software | MOSFLM (CrystFEL) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1894) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.600 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 44284 | |
| <I/σ(I)> | 2.94 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 139.4 | 36.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 298 | 20 mM MgCl2 , 17 %PEG 3,350 |
