4ZI9
Structure of mouse clustered PcdhgA1 EC1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.751, 63.687, 107.486 |
| Unit cell angles | 90.00, 99.69, 90.00 |
Refinement procedure
| Resolution | 33.510 - 1.700 |
| R-factor | 0.2307 |
| Rwork | 0.230 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wcp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.510 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.128 | |
| Number of reflections | 81585 | |
| <I/σ(I)> | 5.42 | 0.33 |
| Completeness [%] | 80.0 | 36 |
| Redundancy | 5.42 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris pH 8.5, 7.5% PEG8000, 200 mM NDSB-221 |
