4ZI6
Crystal structure of leucine aminopeptidase from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.310, 99.430, 99.680 |
| Unit cell angles | 75.26, 61.08, 82.04 |
Refinement procedure
| Resolution | 32.397 - 2.000 |
| R-factor | 0.1732 |
| Rwork | 0.171 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.005 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.142 | 32.397 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.032 | 0.315 | |
| Rmeas | 0.095 | ||
| Rpim | 0.067 | 0.032 | 0.315 |
| Total number of observations | 310850 | 11385 | 37983 |
| Number of reflections | 179957 | ||
| <I/σ(I)> | 7.7 | 18.5 | 2.2 |
| Completeness [%] | 85.1 | 90.7 | 77.3 |
| Redundancy | 1.7 | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 100 mM sodium formate, 11% PEG 3350, 5.6 mg/ml protein |
