4ZHC
Siderocalin-mediated recognition and cellular uptake of actinides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 114.657, 114.657, 119.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.040 |
| R-factor | 0.2074 |
| Rwork | 0.206 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lm6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.8.0049) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.080 |
| High resolution limit [Å] | 2.040 | 5.540 | 2.040 |
| Rmerge | 0.097 | 0.049 | 0.488 |
| Total number of observations | 488919 | ||
| Number of reflections | 51169 | ||
| <I/σ(I)> | 19.2 | ||
| Completeness [%] | 99.7 | 97.6 | 97 |
| Redundancy | 9.6 | 8.7 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 298 | NaCl, Li2SO4, Acetate, Ammonium Sulfate |
