4ZH5

Crystal structure of Endoglucanase from Perinereis brevicirris with Cellobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	76.150, 108.170, 112.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.980 - 1.350
R-factor	0.11665
Rwork	0.115
R-free	0.14812
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wc3
RMSD bond length	0.014
RMSD bond angle	1.610
Data reduction software	MOSFLM
Data scaling software	Aimless (0.3.6)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.980	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.065	0.532
Number of reflections	203725
<I/σ(I)>	9.1	2
Completeness [%]	98.0	97.1
Redundancy	2.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	100 mM Bris-Tris, pH5.5, 300 mM NaCl, 25% PEG 3350