4ZH0
Structure of Helicobacter pylori adhesin BabA determined by SeMet SAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.830, 93.040, 96.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.120 - 1.910 |
| R-factor | 0.19044 |
| Rwork | 0.188 |
| R-free | 0.23039 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.788 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.120 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.236 | 3.430 |
| Number of reflections | 39877 | |
| <I/σ(I)> | 13.2 | 0.9 |
| Completeness [%] | 96.7 | 89.2 |
| Redundancy | 23.1 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 22% PEG 3350, 0.2M Ammonium Acetate, 0.1M Citrate pH 5.6 |
