4ZET

Blood dendritic cell antigen 2 (BDCA-2) complexed with GalGlcNAcMan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 2 21 21
Unit cell lengths	37.559, 67.645, 118.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.560 - 2.900
R-factor	0.182
Rwork	0.178
R-free	0.26300
RMSD bond length	0.013
RMSD bond angle	1.178
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (dev_1760)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.560	37.560	3.070
High resolution limit [Å]	2.900	8.690	2.900
Rmerge	0.146	0.092	0.510
Rpim	0.074	0.048	0.242
Total number of observations	37251	1415	5646
Number of reflections	7094
<I/σ(I)>	8.7	13.7	4.3
Completeness [%]	99.2	98.1	97.4
Redundancy	5.3	4.6	5.2
CC(1/2)	0.985	0.985	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	protein solution comprising 6.2 mg/ml BDCA-2, 5 mM CaCl2, 10 mM Tris-Cl, pH 8.0, 25 mM NaCl and 20 mM GalGlcNAcMan. The reservoir solution contained 0.2 NH4Cl and 20% polyethylene glycol 3.35K