4ZEE
X-ray structure of the bis-platinum lysozyme adduct formed in the reaction between the protein and the two drugs Cisplatin and Oxaliplatin (preparation 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-27 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.377, 77.377, 37.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.710 - 1.950 |
| R-factor | 0.16223 |
| Rwork | 0.159 |
| R-free | 0.22986 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j1a |
| RMSD bond length | 0.020 |
| RMSD bond angle | 4.931 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.710 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.113 | 0.611 |
| Number of reflections | 8452 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 95.8 | 83.2 |
| Redundancy | 5.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | Best crystals grow within 2-10 days from the following conditions: 0.6 M NaNO3, 0.1 M sodium acetate pH 4.4 and 20 % ethylene glycol. |
