4ZDV

Crystal structure of LC3 in complex with FAM134B LIR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	80
Detector technology	PIXEL
Collection date	2013-03-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 42 21 2
Unit cell lengths	62.170, 62.170, 74.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.709 - 1.800
R-factor	0.1861
Rwork	0.184
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eci
RMSD bond length	0.007
RMSD bond angle	1.069
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.170	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.081	0.527
Number of reflections	14123
<I/σ(I)>	17.1
Completeness [%]	100.0
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	8% Tacsimate (Hampton Research) pH 8.0, 20% Poly ethylene glycol 3350