4ZDF
Crystal structure of yeast enoyl-CoA isomerase helix-10 deletion (ScECI2-H10) mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2014-02-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 118.121, 118.121, 87.417 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.940 - 1.810 |
| R-factor | 0.16062 |
| Rwork | 0.159 |
| R-free | 0.18785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k39 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.464 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.940 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.073 | 1.064 |
| Number of reflections | 64106 | |
| <I/σ(I)> | 17.3 | 1.6 |
| Completeness [%] | 99.7 | 97.2 |
| Redundancy | 10.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 100 mM imidazole pH 6.5, 1 M NaAcetate |
