4ZCL
Crystal Structure of Escherichia coli GTPase BipA/TypA Complexed with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.430, 160.010, 90.020 |
| Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
| Resolution | 48.181 - 3.060 |
| R-factor | 0.2342 |
| Rwork | 0.232 |
| R-free | 0.27050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zci |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.741 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_1819) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.181 | 3.250 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.100 | 0.680 |
| Number of reflections | 29871 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 99.3 | 98.8 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris-HCl, 2 % PEG 6000, and 5 mM [Co(NH3)6]Cl3, pH 8.0 |
