4ZCK
Crystal Structure of C-terminal Fragment of Escherichia coli BipA/TypA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 83.562, 83.562, 191.841 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.480 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e3x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.286 |
| Data reduction software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1819) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.580 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.140 | 1.300 |
| Number of reflections | 24915 | |
| <I/σ(I)> | 6.5 | 1 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 7.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 3.04 M sodium formate, 200 mM Tris-HCl, and 1 mM magnesium bromide, pH 7.6 |
