4ZBJ

UBN1 peptide bound to H3.3/H4/Asf1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-18
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	90.024, 90.024, 120.734
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.406 - 2.248
R-factor	0.2095
Rwork	0.208
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hue
RMSD bond length	0.003
RMSD bond angle	0.637
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.406	2.330
High resolution limit [Å]	2.248	2.250
Rmerge	0.054	0.440
Number of reflections	26431
<I/σ(I)>	26.4	2.7
Completeness [%]	99.1	93.3
Redundancy	6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1 M Sodium Cacodylate pH 6.0, 8% PEG 8K, 0.2 M NaCl