4ZB8
Crystal structure of the glutathione transferase URE2P6 from Phanerochaete chrysosporium in complex with oxidized glutathione.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.999 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.848, 77.378, 89.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.088 - 2.000 |
| R-factor | 0.1924 |
| Rwork | 0.192 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zba |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.033 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.168 | 0.609 |
| Number of reflections | 14950 | |
| <I/σ(I)> | 16.9 | 8.2 |
| Completeness [%] | 87.8 | 97.7 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 278 | 20 % PEG10000 0.1M HEPES pH7.5 |
