4ZB1

Crystal Structure of Blue Chromoprotein sgBP from Stichodactyla Gigantea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2014-05-15
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	61.095, 64.772, 154.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.250
R-factor	0.17602
Rwork	0.174
R-free	0.21725
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ib6
RMSD bond length	0.009
RMSD bond angle	1.514
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.095	0.345
Number of reflections	29027
<I/σ(I)>	11.9	3.2
Completeness [%]	96.6	96.5
Redundancy	3.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	24%(w/v) PEG600, 0.2M imidazole malate