4ZB0
A dehydrated form of glucose isomerase collected at room temperature.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2015-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.485, 95.020, 98.484 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.380 - 2.000 |
| R-factor | 0.1393 |
| Rwork | 0.137 |
| R-free | 0.17969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mnz |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.371 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.380 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.138 |
| Total number of observations | 169888 | |
| Number of reflections | 53324 | |
| <I/σ(I)> | 10.4 | 5.9 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Hepes, 10% PEG 400, 0.05 M Manganese Chloride, 20% Glucose |
