4Z9N
ABC transporter / periplasmic binding protein from Brucella ovis with glutathione bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.990, 76.930, 131.470 |
Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
Resolution | 31.655 - 1.745 |
R-factor | 0.1524 |
Rwork | 0.152 |
R-free | 0.18280 |
Structure solution method | SAD |
Starting model (for MR) | phased from iodided soak |
RMSD bond length | 0.008 |
RMSD bond angle | 1.128 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2006: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.745 | 1.745 |
Rmerge | 0.534 | |
Number of reflections | 187580 | |
<I/σ(I)> | 14.4 | 2.8 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | MCSG1b2: 25% PEG3350, 0.2M NaCl, 0.1M BisTris pH 5.5 |