Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 |
| Unit cell lengths | 93.057, 106.058, 114.932 |
| Unit cell angles | 70.12, 84.57, 68.61 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.23620 |
| Starting model (for MR) | 2GL6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.267 |
| Data scaling software | Aimless (0.5.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.090 | 49.090 | 2.030 |
| High resolution limit [Å] | 2.000 | 10.950 | 2.000 |
| Rmerge | 0.077 | 0.019 | 0.897 |
| Rpim | 0.077 | 0.019 | 0.894 |
| Total number of observations | 494536 | 2735 | 24658 |
| Number of reflections | 247985 | ||
| <I/σ(I)> | 8.5 | 36 | 1 |
| Completeness [%] | 95.4 | 90.3 | 95.5 |
| Redundancy | 2 | 1.9 | 2 |
| CC(1/2) | 0.995 | 0.998 | 0.318 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 17% PEG-3350, 10mM DTT, 0.2M diammonium citrate, 0.1M Bis-TRIS, protein buffer also contained magnesium chloride, creatine, ADP |
