4Z9I
Asp-TarS from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2014-06-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.460, 59.510, 79.380 |
Unit cell angles | 90.00, 95.02, 90.00 |
Refinement procedure
Resolution | 42.000 - 1.570 |
R-factor | 0.16793 |
Rwork | 0.166 |
R-free | 0.19849 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2asr |
RMSD bond length | 0.025 |
RMSD bond angle | 2.313 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 1.650 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.058 | |
Number of reflections | 52820 | |
<I/σ(I)> | 18.2 | 6.3 |
Completeness [%] | 99.5 | 98.8 |
Redundancy | 5.4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283 | NaCl |