4Z98
Crystal Structure of Hen Egg White Lysozyme using Serial X-ray Diffraction Data Collection
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2014-12-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03318 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.144, 79.144, 38.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.981 - 1.550 |
R-factor | 0.2089 |
Rwork | 0.205 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vdp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.078 |
Data reduction software | cctbx.xfel |
Phasing software | PHASER |
Refinement software | PHENIX (1.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.590 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 17566 | |
<I/σ(I)> | 14.58 | 3.68 |
Completeness [%] | 96.9 | 91.6 |
Redundancy | 7.9 | 3.44 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 3.5 | 277 | 20% w/v NaCl, 8% w/v PEG 8000, 0.5 M Sodium acetate |