4Z8S
Structural studies on a non-toxic homologue of type II RIPs from Momordica charantia (bitter gourd)-Native-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-10-22 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 144.970, 134.210, 44.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.470 - 2.360 |
R-factor | 0.16652 |
Rwork | 0.165 |
R-free | 0.20144 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hwm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.617 |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.470 | 2.490 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.110 | |
Number of reflections | 36839 | |
<I/σ(I)> | 12.2 | 3.18 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1M HEPES, 20% w/v PEG 10000 |