4Z8C

Crystal structure of the Thermus thermophilus 70S ribosome bound to translation inhibitor oncocin

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	210.140, 450.730, 623.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.981 - 2.900
R-factor	0.2051
Rwork	0.203
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.717
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.185	0.032	2.112
Rmeas	0.213	0.037	2.466
Total number of observations	5417877
Number of reflections	1407992	15416	99263
<I/σ(I)>	7.03	35.8	0.66
Completeness [%]	98.2	90.9	94
Redundancy	3.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 7-12% MPD, 0.5 mM BME