4Z88
SH3-II of Drosophila Rim-binding protein with Aplip1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.280, 62.420, 163.640 |
| Unit cell angles | 90.00, 90.27, 90.00 |
Refinement procedure
| Resolution | 45.250 - 2.090 |
| R-factor | 0.21005 |
| Rwork | 0.209 |
| R-free | 0.23642 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2csq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.224 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.100 | 0.400 |
| Number of reflections | 64269 | |
| <I/σ(I)> | 7.7 | 2.6 |
| Completeness [%] | 98.9 | 92.4 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 100 mM Bicine, 2.2-2.6 M Ammoniumsulfate |
