4Z86
Crystal structure of Peptidyl-tRNA hydrolase mutant -N118D from Vibrio cholerae at 1.63A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-12-29 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.112, 71.124, 124.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.630 |
| R-factor | 0.17201 |
| Rwork | 0.170 |
| R-free | 0.20690 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.010 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 49251 | |
| <I/σ(I)> | 51.92 | 4.68 |
| Completeness [%] | 98.9 | 95.7 |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 100mM Soduim citrate, 200mM Ammonium acetate, 25% Polyethylene glycol 4000 |
