4Z7F

Crystal structure of FolT bound with folic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	77.15
Detector technology	CCD
Collection date	2013-07-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 31
Unit cell lengths	92.752, 92.752, 183.445
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.569 - 3.194
R-factor	0.299
Rwork	0.293
R-free	0.35640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4huq
RMSD bond length	0.017
RMSD bond angle	2.470
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	3.310
High resolution limit [Å]	3.194	6.880	3.200
Rmerge	0.138	0.056
Rmeas	0.150	0.067
Rpim	0.093	0.036	0.855
Total number of observations	101633
Number of reflections	28978
<I/σ(I)>	6.3
Completeness [%]	98.3	94.8	99.3
Redundancy	3.5	3.2	3.5
CC(1/2)		0.995	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293.15	15% (w/v) polyethylene glycol 2000, 0.5M NaCl, 0.1M sodium dihydrogen phosphate