4Z6Y
Structure of the TBC1D7-TSC1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.991, 145.689, 114.758 |
| Unit cell angles | 90.00, 89.65, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.810 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.25500 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.715 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 120.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 44658 |
| <I/σ(I)> | 12.3 |
| Completeness [%] | 98.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 8% PEG 6000, 4% 2-methyl-2,4-pentanediol (MPD), 0.1M HEPES, pH7.5 |
