4Z62
The plant peptide hormone free receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-01 |
Detector | BRUKER SMART 6500 |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 90.033, 98.790, 227.294 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.378 - 2.900 |
R-factor | 0.205 |
Rwork | 0.202 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.449 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 21402 |
<I/σ(I)> | 18.5 |
Completeness [%] | 93.6 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris 8.5, 2.0 M (NH4)2SO4 |