4Z5X

Glycogen phosphorylase in complex with gallic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	CCD
Collection date	2013-12-07
Detector	RAYONIX MX225HE
Wavelength(s)	1.04
Spacegroup name	P 43 21 2
Unit cell lengths	128.486, 128.486, 116.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.850 - 2.100
R-factor	0.16058
Rwork	0.159
R-free	0.18928
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.337
Data reduction software	MOSFLM
Data scaling software	XSCALE
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.360	2.210
High resolution limit [Å]	2.100	2.100
Number of reflections	56323
<I/σ(I)>	15.4	5.1
Completeness [%]	98.4	97.8
Redundancy	6.7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.8	289	10 mM BES BUFFER