4Z4U
Crystal structure of GII.10 P domain in complex with 37.5mM fucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-20 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.984463 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.317, 78.774, 68.512 |
Unit cell angles | 90.00, 99.52, 90.00 |
Refinement procedure
Resolution | 43.100 - 1.890 |
R-factor | 0.1592 |
Rwork | 0.157 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3onu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.062 |
Data reduction software | XDS |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.150 | 1.953 |
High resolution limit [Å] | 1.885 | 1.885 |
Rmerge | 0.078 | 0.608 |
Number of reflections | 54715 | |
<I/σ(I)> | 11.56 | 1.81 |
Completeness [%] | 98.9 | 95.03 |
Redundancy | 3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | sodium nitrate, bis-tris propane, PEG3350 |