4Z4T
Crystal structure of GII.10 P domain in complex with 75mM fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.984463 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.749, 78.850, 68.642 |
| Unit cell angles | 90.00, 99.43, 90.00 |
Refinement procedure
| Resolution | 43.419 - 1.800 |
| R-factor | 0.1491 |
| Rwork | 0.148 |
| R-free | 0.17370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3onu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.420 | 1.863 |
| High resolution limit [Å] | 1.798 | 1.798 |
| Rmerge | 0.044 | 0.187 |
| Number of reflections | 63508 | |
| <I/σ(I)> | 18.25 | 5.97 |
| Completeness [%] | 98.8 | 96.05 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | sodium nitrate, bis-tris propane, PEG3350 |
