4Z4H

Human Argonaute2 A481T Mutant Bound to t1-A Target RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99999
Spacegroup name	P 1 21 1
Unit cell lengths	55.695, 116.602, 70.099
Unit cell angles	90.00, 92.29, 90.00

Refinement procedure

Resolution	44.810 - 2.504
R-factor	0.174
Rwork	0.172
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4w5o
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.810	2.610
High resolution limit [Å]	2.500	2.500
Rmerge		0.127
Number of reflections	30411
<I/σ(I)>	9.2
Completeness [%]	98.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Tris, Isopropanol, Phenol, Magnesium