4Z4E

Human Argonaute2 Bound to t1-U Target RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	55.645, 116.841, 69.744
Unit cell angles	90.00, 92.43, 90.00

Refinement procedure

Resolution	40.274 - 1.800
R-factor	0.1589
Rwork	0.158
R-free	0.18540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4w5o
RMSD bond length	0.007
RMSD bond angle	1.131
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.600	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.098
Number of reflections	82071
<I/σ(I)>	10.3
Completeness [%]	99.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Tris, Isopropanol, Phenol, Magnesium