4Z4E
Human Argonaute2 Bound to t1-U Target RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.645, 116.841, 69.744 |
| Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
| Resolution | 40.274 - 1.800 |
| R-factor | 0.1589 |
| Rwork | 0.158 |
| R-free | 0.18540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w5o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.600 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.098 | |
| Number of reflections | 82071 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, Tris, Isopropanol, Phenol, Magnesium |
