4Z3N
Crystal structure of the MATE transporter ClbM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.460, 79.460, 174.921 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.730 - 2.700 |
| R-factor | 0.2114 |
| Rwork | 0.206 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vvn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.157 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.730 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.104 | 0.554 |
| Number of reflections | 17649 | |
| <I/σ(I)> | 8.5 | 2.6 |
| Completeness [%] | 96.6 | 89.4 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.8 | 293 | PEG 500 DME, 100 mM lithium sulfate, 400 mM sodium chloride, 100 mM sodium cacodylate |
