4Z2Y
Crystal structure of methyltransferase CalO6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.007 |
Spacegroup name | H 3 2 |
Unit cell lengths | 126.828, 126.828, 105.659 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.400 |
R-factor | 0.32502 |
Rwork | 0.325 |
R-free | 0.33338 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.444 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.100 |
Number of reflections | 11759 |
<I/σ(I)> | 28 |
Completeness [%] | 98.1 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 294 | Reservoir: 0.1 M NaCl, 0.1 M Bis-Tris (pH 5.8), and 1.10-1.25 M ammonium sulfate |