4Z28
Crystal structure of short hoefavidin biotin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.180, 61.180, 166.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.660 |
R-factor | 0.18194 |
Rwork | 0.180 |
R-free | 0.23017 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4z27 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.791 |
Data reduction software | MOSFLM |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.720 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.045 | 0.798 |
Number of reflections | 38372 | |
<I/σ(I)> | 19.9 | |
Completeness [%] | 99.8 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Streak seeding was applied with source crystals. Crystals appeared within 24 hours and grew to their maximal size within 2 weeks |