4Z1V

Structure of Factor Inhibiting HIF (FIH) in complex with Fe, NO, and NOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	86.650, 86.650, 147.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.740 - 2.100
R-factor	0.1945
Rwork	0.190
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d8c
RMSD bond length	0.019
RMSD bond angle	1.890
Data reduction software	MOSFLM
Data scaling software	SCALA (0.2.8)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.740	61.270	2.170
High resolution limit [Å]	2.100	8.400	2.100
Rmerge	0.097	0.032	1.092
Rpim	0.025	0.009	0.369
Total number of observations	503994	7417	26630
Number of reflections	33489
<I/σ(I)>	16	40.4	1.9
Completeness [%]	99.6	92.4	96.8
Redundancy	15	12.8	9.1
CC(1/2)	0.999	0.999	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Hepes, 1.6mM ammonium sulfate, 3% PEG 400