4Z1L
Yeast 20S proteasome in complex with belactosin C derivative 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 136.085, 301.299, 144.582 |
| Unit cell angles | 90.00, 113.33, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.20536 |
| Rwork | 0.204 |
| R-free | 0.22907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.899 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.040 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.118 | 0.499 |
| Number of reflections | 207849 | |
| <I/σ(I)> | 10.7 | 1.9 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |
