4YYV
Ficin isoform C crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.471, 52.471, 157.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.643 - 1.902 |
| R-factor | 0.1769 |
| Rwork | 0.173 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yyr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.414 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.643 | 1.960 |
| High resolution limit [Å] | 1.902 | 1.900 |
| Rmerge | 0.097 | 0.599 |
| Number of reflections | 18158 | |
| <I/σ(I)> | 18.5 | 4.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.9 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG8000, 0.1M Na-Cacodylate, 0.2 M (NH4)2SO4 |
