4YYK
Crystal structure of BRD9 Bromodomain bound to a crotonyllysine peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 |
| Unit cell lengths | 24.840, 34.730, 129.080 |
| Unit cell angles | 89.88, 89.81, 69.45 |
Refinement procedure
| Resolution | 32.270 - 1.790 |
| R-factor | 0.2271 |
| Rwork | 0.225 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hme |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.846 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 3.620 | 1.680 |
| Rmerge | 0.089 | 0.077 | 0.617 |
| Total number of observations | 88233 | ||
| Number of reflections | 36333 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 96.6 | 93.5 | 99.9 |
| Redundancy | 3.7 | 4.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M MES, pH 6.5, 15% w/v PEG550 MME |
